GeneTex
United States (US)

ERAB antibody [5F3]

ERAB-antibody-5F3__GTX10260-1_19011506_715.jpg
ERAB-antibody-5F3-GTX10260-ICC_IF-1_19011506_196.jpg

Cat No. GTX10260

Host Mouse
Clonality Monoclonal
Clone Name 5F3
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr, Dot, ELISA
Reactivity Human
APPLICATION

Application Note

Dot: Use at a dilution of 1/100 - 1/10000. ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/100 - 1/1000. IHC-Fr: Use at a dilution of 1/100 - 1/1000. WB: Use at a dilution of 1/100 - 1/10000. Predicted molecular weight: 27 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

27 kDa. ( Note )

Specificity/Sensitivity

This antibody detects the recombinant human ERAB protein (27kDa) and recognizes the endogenous ERAB protein in cell extracts with virtually no crossreactivity with other human proteins.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, no preservative added.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant, full-length Human ERAB protein (fusion protein).

Purification


Ammonium sulfate precipitated and dialyzed tissue culture supernatant.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ABAD, 300256, MHBD, 3028, 17bHSD10, HSD17B10, 17b-HSD10, HADH2, ERAB, Q99714, 17b HSD10

Cellular Localization

Mitochondrial

Background

ERAB (Endoplasmic Reticulum beta Amyloid Binding Protein) has been associated with beta Amyloid neurotoxicity. ERAB is an intracellular 27 kDa polypeptide which appears to be upregulated in the brains of Alzheimers Disease patients.

Database

Research Area

DATA IMAGES
ERAB-antibody-5F3__GTX10260-1_19011506_715.jpg

GTX10260 ICC/IF Image

Mitochondrial localization of ERAB in human neuroblastoma cells SK-N-SH visualized with MAb 5F3 (GTX10260)) and laser-scanning confocal microscope. 
A. MAb to mitochondria visualized with Cy-5-conjugated secondary antibodies
B. MAb 5F3 to ERAB-FITC-conjugated
C. Merged image

ERAB-antibody-5F3-GTX10260-ICC_IF-1_19011506_196.jpg

GTX10260 ICC/IF Image

IF using GTX10260


Package List Price ($)
$ 319