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ERCC1 antibody

KO/KD Orthogonal
Anti-ERCC1 antibody used in Western Blot (WB). GTX129282
Anti-ERCC1 antibody used in Western Blot (WB). GTX129282
Anti-ERCC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129282
Anti-ERCC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129282
Anti-ERCC1 antibody used in Western Blot (WB). GTX129282

Cat. No. GTX129282

Reference 2
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ERCC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , PC-12 , Rat2

Predict Reactivity

Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERCC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885953

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ERCC excision repair 1, endonuclease non-catalytic subunit , COFS4 , RAD10 , UV20

Cellular Localization

Nucleus

Background

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ERCC1 antibody used in Western Blot (WB). GTX129282

GTX129282 WB Image

KO/KD

Wild-type (WT) and ERCC1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERCC1 antibody (GTX129282) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERCC1 antibody used in Western Blot (WB). GTX129282

GTX129282 WB Image

ERCC1 antibody detects ERCC1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
ERCC1 antibody (GTX129282) dilution: 1:1000

Anti-ERCC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129282

GTX129282 IHC-P Image

ERCC1 antibody detects ERCC1 protein at cytoplasm and nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
ERCC1 antibody (GTX129282) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERCC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129282

GTX129282 ICC/IF Image

ERCC1 antibody detects ERCC1 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERCC1 protein stained by ERCC1 antibody (GTX129282) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-ERCC1 antibody used in Western Blot (WB). GTX129282

GTX129282 WB Image

ERCC1 antibody detects ERCC1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
ERCC1 antibody (GTX129282) dilution: 1:1000

REFERENCE

Application Reference

Kiminori Kurashima et al. NAR Cancer 2020; 2 (2) : zcaa005 SMARCA4 deficiency-associated heterochromatin induces intrinsic DNA replication stress and susceptibility to ATR inhibition in lung adenocarcinoma.
Application : WB
Reactivity : Human
Kawara H et al. Biochem Biophys Res Commun 2019; (Epub) DCAF7 is required for maintaining the cellular levels of ERCC1-XPF and nucleotide excision repair.
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REVIEW

ERCC1 antibody Cat. No. GTX129282

Rating
( Average 4 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4 based on 2 users reviews)
Anti-ERCC1 antibody used in Western Blot (WB). GTX129282
Date : Anonymous submitted on 20-Dec-2022
Score :
Application Tested : WB
Sample Species : Ms
Sample : CT26
Amount used : 30ug
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Anti-ERCC1 antibody used in Western Blot (WB). GTX129282
Date : Anonymous submitted on 24-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 8μg
Blocking : 10% BSA, RT, 1Hr
Primary Antibody : 1:1000, 4°C, 8Hr
Figure Note : The observed M.W. is based on the publication: PMID: 29447131.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169