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ERCC8 antibody [N2C2], Internal

Anti-ERCC8 antibody [N2C2], Internal used in Western Blot (WB). GTX100145
Anti-ERCC8 antibody [N2C2], Internal used in Western Blot (WB). GTX100145
Anti-ERCC8 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100145

Cat. No. GTX100145

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, Functional Assay, PLA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ERCC8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Functional Assay Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Neuro2A , GL261 , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERCC8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950221

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ERCC excision repair 8, CSA ubiquitin ligase complex subunit , CKN1 , CSA , UVSS2

Cellular Localization

Nucleus

Background

This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ERCC8 antibody [N2C2], Internal used in Western Blot (WB). GTX100145

GTX100145 WB Image

ERCC8 antibody [N2C2], Internal detects ERCC8 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
F. 30 μg C2C12 whole cell lysate/extract
10 % SDS-PAGE
ERCC8 antibody [N2C2], Internal (GTX100145) dilution: 1:1000

Anti-ERCC8 antibody [N2C2], Internal used in Western Blot (WB). GTX100145

GTX100145 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERCC8 antibody [N2C2], Internal (GTX100145) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERCC8 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100145

GTX100145 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ERCC8(CSA)(GTX100145) antibody at 1:200 dilution.

REFERENCE

Application Reference

E Paccosi et al. Eur J Cell Biol 2023; 102 (2) : 151325 Cockayne syndrome group A protein localizes at centrosomes during mitosis and regulates Cyclin B1 ubiquitination
Application : ICC/IF
Donnio LM et al. Elife 2022; 11 XAB2 dynamics during DNA damage-dependent transcription inhibition.
Application : PLA
Reactivity : Human
Lanzafame M et al. Nucleic Acids Res 2021; 49 (19) : 10911-10930 Cockayne syndrome group A and ferrochelatase finely tune ribosomal gene transcription and its response to UV irradiation.
Application : WB
Reactivity : Human
Dubois EL et al. Oncol Rep 2016; 35 (5) : 1-May UV-dependent phosphorylation of COP9/signalosome in UV-induced apoptosis.
Fischer ES et al. Cell 2011; 147 (5) : 1024-39 The molecular basis of CRL4DDB2/CSA ubiquitin ligase architecture, targeting, and activation.
Application : Functional Assay
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169
Currently in production