GeneTex
United States (US)

ERp57 antibody

KO/KD Overexpression
GTX113719_40471_20150410_IFA_18121410_562.jpg
GTX113719_40471_WB_18121410_314.jpg
GTX113719_40471_20150605_WB_18121410_203.jpg
GTX113719_40471_WB_M_18121410_358.jpg
GTX113719_40471_20160421_WB_R_18121410_136.jpg
GTX113719_40471_IHC_18121410_882.jpg
GTX113719_40471_20141231_IHC_M_18121410_474.jpg

Cat No. GTX113719

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HepG2 , HCT116 , 293T , A431 , HeLa , Neuro2A , GL261 , C8D30 , PC-12 , Rat2

Specificity/Sensitivity

Knockdown/Knockout & overexpression validation were supported by customer review data.

Predict Reactivity

Rabbit, Sheep, Bovine, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERp57. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ER60 antibody, ERp57 antibody, ERp60 antibody, ERp61 antibody, GRP57 antibody, GRP58 antibody, HsT17083 antibody, P58 antibody, PI-PLC antibody, PDIA3 antibody, protein disulfide isomerase-associated 3 antibody, ER protein 60 antibody, 58 kDa glucose-regulated protein antibody, endoplasmic reticulum resident protein 60 antibody, phospholipase C-alpha antibody, ER protein 57 antibody, endoplasmic reticulum P58 antibody, "glucose regulated protein, 58kDa antibody", disulfide isomerase ER-60 antibody, endoplasmic reticulum resident protein 57 antibody, protein disulfide-isomerase A3 antibody, 58 kDa microsomal protein antibody, "protein disulfide isomerase family A, member 3 antibody"

Cellular Localization

Endoplasmic reticulum lumen (By similarity) , Melanosome

Background

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX113719_40471_20150410_IFA_18121410_562.jpg

GTX113719 ICC/IF Image

ERp57 antibody detects ERp57 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERp57 protein stained by ERp57 antibody (GTX113719) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX113719_40471_WB_18121410_314.jpg

GTX113719 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
7.5% SDS PAGE
GTX113719 diluted at 1:10000

GTX113719_40471_20150605_WB_18121410_203.jpg

GTX113719 WB Image

ERp57 antibody detects ERp57 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERp57 antibody (GTX113719) diluted by 1:10000.

GTX113719_40471_WB_M_18121410_358.jpg

GTX113719 WB Image

Sample (30 ug of whole cell lysate)
A: Neuro2A
B: GL261
C: C8D30
7.5% SDS PAGE
GTX113719 diluted at 1:1000

GTX113719_40471_20160421_WB_R_18121410_136.jpg

GTX113719 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody (GTX113719) diluted at 1:500.

GTX113719_40471_IHC_18121410_882.jpg

GTX113719 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ERp57(GTX113719) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113719_40471_20141231_IHC_M_18121410_474.jpg

GTX113719 IHC-P Image

ERp57 antibody detects ERp57 protein at cytoplasm on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
ERp57 antibody (GTX113719) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

ERp57 antibody Cat No. GTX113719

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 02-Nov-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T, HeLa cells
Amount used : 10 μg
Blocking : Blocking One, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, overnightHr
Date : Anonymous submitted on 27-Nov-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Caco2 cells
Amount used : 20μg
Blocking : 5% milk in PBST, 25°C, 1.5Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf