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ETO / RUNX1T1 antibody - ChIP grade

Anti-ETO / RUNX1T1 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60332
Anti-ETO / RUNX1T1 antibody - ChIP grade used in ELISA (ELISA). GTX60332
Anti-ETO / RUNX1T1 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60332

Cat. No. GTX60332

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ELISA, ChIP assay

Reactivity

 Human
Package
50 μl ($399)
See all ETO / RUNX1T1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ELISA 1:100
ChIP assay 1-10 μl
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Human ETO (runt-related transcription factor 1; translocated to, 1 (cyclin D-related)) using two KLH-conjugated synthetic peptides containing sequences from the N-terminal and the central region of the protein, respectively.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RUNX1 translocation partner 1 , AML1-MTG8 , AML1T1 , CBFA2T1 , CDR , ETO , MTG8 , ZMYND2

Cellular Localization

Nucleus

Background

This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]

Database

  • Gene ID: 862 RUNX1T1
  • UniProt: Q06455 RUNX1T1

Research Area

DATA IMAGES

Anti-ETO / RUNX1T1 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60332

GTX60332 ChIP assay Image

ChIP analysis of SKNO-1 cells using GTX60332 ETO / RUNX1T1 antibody - ChIP grade. The IP'd DNA of 6 ChIP's was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.

Anti-ETO / RUNX1T1 antibody - ChIP grade used in ELISA (ELISA). GTX60332

GTX60332 ELISA Image

ELISA analysis of peptides used for immunization using GTX60332 ETO / RUNX1T1 antibody - ChIP grade.

Anti-ETO / RUNX1T1 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60332

GTX60332 ChIP assay Image

ChIP analysis of sheared chromatin from 1.25x10⁶ SKNO-1 cells using GTX60332 ETO / RUNX1T1 antibody - ChIP grade. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. This figure shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used.
Antibody amount : 4μl

REFERENCE

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REVIEW

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Package List Price ($)
$ 399