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Ebola virus NP antibody

Anti-Ebola virus NP antibody used in Western Blot (WB). GTX134031

Cat. No. GTX134031

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Ebola virus
Package
100 μl ($399),
25 μl ($169)
See all Ebola virus NP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Positive Control

DDDDK-tagged Zaire ebolavirus NP-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Ebola virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Ebola virus NP protein (Zaire ebolavirus (strain Mayinga-76)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887180

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Ebola virus Nucleoprotein , Ebola virus NP , EBOV Nucleoprotein , EBOV NP

Research Area

DATA IMAGES

Anti-Ebola virus NP antibody used in Western Blot (WB). GTX134031

GTX134031 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ebola virus NP antibody (GTX134031) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Vidal S et al. mBio 2021; 12 (4) : e0097221 Expression of the Ebola Virus VP24 Protein Compromises the Integrity of the Nuclear Envelope and Induces a Laminopathy-Like Cellular Phenotype.
Application : WB
Submit a Reference

REVIEW

Ebola virus NP antibody Cat. No. GTX134031

Rating
( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Ebola virus NP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134031
Date : Reika Watanabe submitted on 13-Jan-2022
Score :
Application Tested : ICC/IF
Sample : HEK293T expressing Ebola Zaire NP
Fixation : -20 degree Methanol
Permeabilization /Antigen retrieval : no additional permeabilization
Primary Antibody : 1:300, room temperature°C, 1Hr
Figure Note : HEK293T cells transiently expressing Ebola Zaire NP and control non-transfected HEK293T cells were fixed by cold methanol (-20 degree). The cells were stained with primary anti-NP antibody (1:300) at room temperature for 1 hr followed by secondary antibody (anti-Rabbit conjugated with Alexa 488). Images are observed by wide-filed fluorescence microscopy using a 40 x objective lens.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169