GeneTex
United States (US)

Ef1a antibody

GTX124537_40100_WB_Z_18121410_399.jpg
GTX124537_40100_IHC_Z_18121410_759.jpg

Cat No. GTX124537

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

zebrafish eye , zebrafish embryo , zebrafish kidney
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Elongation Factor 1 Alpha 1, Like 1,Efl1-Alpha,Chunp6927,Eef1A,Ef1A,Ik:Tdsubc_2A3,Ik:Tdsubc_2B3,Tdsubc_2A3,Wu:Fa91C07,Wu:Fa94B03,Wu:Fi13B09,Xx:Tdsubc_2A3,Xx:Tdsubc_2B3,Eef1A1L1

Cellular Localization

Cytoplasm

Background

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Database

Research Area

DATA IMAGES
GTX124537_40100_WB_Z_18121410_399.jpg

GTX124537 WB Image

Sample (30 µg of whole cell lysate)
A: zebrafish eye
B: zebrafish embryo
C: zebrafish kidney
10% SDS PAGE
GTX124537 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124537_40100_IHC_Z_18121410_759.jpg

GTX124537 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ef1a (GTX124537) antibody at 1:300 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 389
$ 199