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Ef1a antibody

Anti-Ef1a antibody used in Western Blot (WB). GTX124538
Anti-Ef1a antibody used in IHC (Paraffin sections) (IHC-P). GTX124538

Cat. No. GTX124538

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Ef1a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

zebrafish embryo

Predict Reactivity

Human (100%)| Mouse (99%)| Rat (99%)| Zebrafish (95%)| Rabbit (100%)| Bovine (100%)| Chicken (99%)| Japanese medaka (80%)| Xenopus tropicalis (98%)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Ef1a

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177741

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation elongation factor 1 alpha 1, like 1 , EFL1-alpha , chunp6927 , eef1a , ef1a , ik:tdsubc_2a3 , ik:tdsubc_2b3 , tdsubc_2a3 , wu:fa91c07 , wu:fa94b03 , wu:fi13b09 , xx:tdsubc_2a3 , xx:tdsubc_2b3

Cellular Localization

Cytoplasm

Background

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Database

Research Area

DATA IMAGES

Anti-Ef1a antibody used in Western Blot (WB). GTX124538

GTX124538 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish embryo
10% SDS PAGE
GTX124538 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Ef1a antibody used in IHC (Paraffin sections) (IHC-P). GTX124538

GTX124538 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ef1a (GTX124538) antibody at 1:300 dilution.

REFERENCE

Application Reference

Mostowy S et al. PLoS Pathog 2013; 9 (9) : e1003588 The zebrafish as a new model for the in vivo study of Shigella flexneri interaction with phagocytes and bacterial autophagy.
Application : WB
Reactivity : Zebrafish
Submit a Reference

REVIEW

Ef1a antibody Cat. No. GTX124538

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-Ef1a antibody used in Western Blot (WB). GTX124538
Date : Anonymous submitted on 14-Aug-2013
Score :
Application Tested : WB
Sample Species : Zfsh
Sample : Liver lysate of adult zebrafish
Amount used : 5μg
Blocking : 5% skim milk in PBST, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169