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Elavl3+4 antibody

Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365
Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365
Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365
Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365
Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365
Anti-Elavl3+4 antibody used in IHC (Frozen sections) (IHC-Fr). GTX128365

Cat. No. GTX128365

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF, IHC-Fr, IHC-Wm

Reactivity

Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Predict Reactivity

Mouse, Rat, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Elavl3+4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885764

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Elavl3 antibody , HuD antibody , HuC/D antibody , HuC antibody , wu:fb77b03 antibody , id:ibd1248 antibody , elrc antibody , zHuC antibody , ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) antibody , ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) antibody

Background

Implicated in tumor angiogenesis (By similarity). May inhibits BMPs activity by blocking their interaction with their receptors. Has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal cells, by preventing or reducing the rate of matrix accumulation. May play a role during myoblast and osteoblast differentiation, and maturation.

Research Area

DATA IMAGES

Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365

GTX128365 IHC-Wm Image

Elavl3+4 antibody detects Elavl3+4 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Elavl3+4 stained by Elavl3+4 antibody (GTX128365) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365

GTX128365 IHC-Wm Image

Elavl3+4 antibody and Tubulin (Acetyl Lys40) antibody label central nervous system on zebrafish by whole mount immunohistochemical analysis.
Sample: 24 hours-post-fertilization zebrafish embryo.
Green: Elavl3+4 antibody (GTX128365) dilution: 1:100.
Red: Tubulin (Acetyl Lys40) antibody [6-11B-2] (GTX16292) dilution: 1:25.

Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365

GTX128365 IHC-Wm Image

Elavl3+4 antibody and Tubulin (Acetyl Lys40) antibody label central nervous system on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Green: Elavl3+4 antibody (GTX128365) dilution: 1:100.
Red: Tubulin (Acetyl Lys40) antibody [6-11B-2] (GTX16292) dilution: 1:25.

Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365

GTX128365 IHC-Wm Image

Elavl3+4 antibody detects Elavl3+4 protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Elavl3+4 antibody (GTX128365) dilution: 1:200.

Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365

GTX128365 IHC-Wm Image

Elavl3+4 antibody and Tubulin (Acetyl Lys40) antibody label central nervous system on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Green: Elavl3+4 antibody (GTX128365) dilution: 1:100.
Red: Tubulin (Acetyl Lys40) antibody [6-11B-2] (GTX16292) dilution: 1:25.

Anti-Elavl3+4 antibody used in IHC (Frozen sections) (IHC-Fr). GTX128365

GTX128365 IHC-Fr Image

The data was published in the journal Brain Struct Funct in 2018.PMID: 29423637

REFERENCE

REVIEW

Elavl3+4 antibody Cat. No. GTX128365

Rating ( Average 4 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 17-Jul-2019
Score :
Application Tested : IHC-Fr
Sample : Adult mouse brain tissue
Blocking : 1% BSA in PBS, 25°C, 0.5Hr
Fixation : 4% praformaldehyde in PBS
Permeabilization /Antigen retrieval : 0.3% Triton X -100 in PBS
Primary Antibody : 1:200, 4°C, 16Hr
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169