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Enterovirus 71 VP1 antibody

Orthogonal
Anti-Enterovirus 71 VP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132339
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132339
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132339
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132339
Anti-Enterovirus 71 VP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132339
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132339
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132339
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132339

Cat. No. GTX132339

Reference 15

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Enterovirus 71
Package
100 μl ($399),
25 μl ($169)
See all Enterovirus 71 VP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

EV71 C2 infected RD , EV71 B4 infected RD

Product Note

This antibody was raised against Enterovirus 71 VP1, and it does not cross-react with Coxsackievirus A16.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Enterovirus 71

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Enterovirus 71 VP1 (EV71 virus (strain USA/BrCr/1970)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886617

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Enterovirus 71 Capsid protein VP1 , Enterovirus 71 VP1 , EV71 Capsid protein VP1 , EV71 VP1

Research Area

DATA IMAGES

Anti-Enterovirus 71 VP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132339

GTX132339 IHC-P Image

EV71 VP1 antibody detects EV71 VP1 protein in EV71 infected mouse skeletal muscle by immunohistochemical analysis. Sample: Paraffin-embedded EV71 infected mouse skeletal muscle. EV71 VP1 antibody [GTX132339] diluted at 1:500
Antigen Retrieval: Citrate buffer

Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132339

GTX132339 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody (GTX132339) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132339

GTX132339 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody (GTX132339) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132339

GTX132339 WB Image

Non-infected (–) and infected RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody (GTX132339) diluted at 1:5000.

Anti-Enterovirus 71 VP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132339

GTX132339 ICC/IF Image

EV71 VP1 antibody detects EV71 VP1 protein by immunofluorescent analysis. Sample: RD cell infected with EV71 were fixed in 4% paraformaldehyde. Green: EV71 VP1 protein stained by EV71 VP1 antibody (GTX132339) diluted at 1:100. Blue: DAPI staining. Scale bar = 500 μm.

Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132339

GTX132339 WB Image

Orthogonal

The data was published in the journal Viruses in 2020.PMID: 32276428

Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132339

GTX132339 WB Image

Orthogonal

The data was published in the journal Viruses in 2020.PMID: 32276428

Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132339

GTX132339 WB Image

The data was published in the journal Viruses in 2020.PMID: 32276428

REFERENCE

Application Reference

Meng Y et al. Viruses 2023; 15 (10) Umbilical Cord Mesenchymal-Stem-Cell-Derived Exosomes Exhibit Anti-Oxidant and Antiviral Effects as Cell-Free Therapies.
Y Meng et al. Viruses 2023; Umbilical Cord Mesenchymal-Stem-Cell-Derived Exosomes Exhibit Anti-Oxidant and Antiviral Effects as Cell-Free Therapies
Xie H et al. Virus Res 2023; 330 : 199108 Targeting HDAC11 activity by FT895 restricts EV71 replication.
Ji W et al. PLoS Negl Trop Dis 2023; 17 (1) : e0011001 TBK1 and IRF3 are potential therapeutic targets in Enterovirus A71-associated diseases.
Application : IHC-P
Ruan Z et al. Front Microbiol 2022; 13 : 1024899 Enterovirus 71 non-structural protein 3A hijacks vacuolar protein sorting 25 to boost exosome biogenesis to facilitate viral replication.
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REVIEW

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Application Reference
Meng Y et al. Viruses 2023; 15 (10) Umbilical Cord Mesenchymal-Stem-Cell-Derived Exosomes Exhibit Anti-Oxidant and Antiviral Effects as Cell-Free Therapies.
Y Meng et al. Viruses 2023; Umbilical Cord Mesenchymal-Stem-Cell-Derived Exosomes Exhibit Anti-Oxidant and Antiviral Effects as Cell-Free Therapies
Xie H et al. Virus Res 2023; 330:199108 Targeting HDAC11 activity by FT895 restricts EV71 replication.
Ji W et al. PLoS Negl Trop Dis 2023; 17 (1):e0011001 TBK1 and IRF3 are potential therapeutic targets in Enterovirus A71-associated diseases.
Application : IHC-P
Ruan Z et al. Front Microbiol 2022; 13:1024899 Enterovirus 71 non-structural protein 3A hijacks vacuolar protein sorting 25 to boost exosome biogenesis to facilitate viral replication.
Jiaoyan Jia et al. Front Microbiol 2022; 13:865644 Heat Shock Protein A6 Is Especially Involved in Enterovirus 71 Infection.
Application : WB, ICC/IF
Zhang X et al. Mol Ther 2020; 28 (6):1533-1546 Enterovirus A71 Oncolysis of Malignant Gliomas.
Application : ICC/IF
Su W et al. Viruses 2020; 12 (4) Interaction between PHB2 and Enterovirus A71 VP1 Induces Autophagy and Affects EV-A71 Infection.
Saeed M et al. PLoS Pathog 2020; 16 (9):e1008927 Defining the proteolytic landscape during enterovirus infection.
Application : ICC/IF
Feng Q et al. Infect Genet Evol 2020; 81:104210 Acarbose, as a potential drug, effectively blocked the dynamic metastasis of EV71 from the intestine to the whole body.
Application : WB, IHC-P
Fan S et al. NPJ Vaccines 2019; 4:14 Role of innate lymphoid cells and dendritic cells in intradermal immunization of the enterovirus antigen.
Chen SL et al. Emerg Microbes Infect 2019; 8 (1):773-786 Endophilin-A2-mediated endocytic pathway is critical for enterovirus 71 entry into caco-2 cells.
Application : ICC/IF
Cao J et al. Microb Pathog 2019; 134:103568 Determination of the cleavage site of enterovirus 71 VP0 and the effect of this cleavage on viral infectivity and assembly.
Application : WB
Jin Y et al. Lab Invest 2018; (Epub) Mast cells contribute to Enterovirus 71 infection-induced pulmonary edema in neonatal mice.
Application : ICC/IF
Yen MH et al. Kaohsiung J Med Sci. 2017; (Epub) Artemisia capillaris inhibited enterovirus 71-induced cell injury by preventing viral internalization.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169