Enterovirus 71 VP3 antibody

Name: Enterovirus 71 VP3 antibody
Brand: GeneTex
SKU: GTX132341
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Enterovirus 71 VP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132341

Anti-Enterovirus 71 VP3 antibody used in Western Blot (WB). GTX132341

Cat. No. GTX132341

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Enterovirus 71
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX132341 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000

Not tested in other applications.

Positive Control

EV71-infected RD cell

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Enterovirus 71

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Enterovirus 71 VP3 (EV71 virus (strain USA/BrCr/1970)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886619

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Enterovirus 71 Capsid protein VP3 , Enterovirus 71 VP3 , EV71 Capsid protein VP3 , EV71 VP3

Research Area

DATA IMAGES

GTX132341 ICC/IF Image

EV71 VP3 antibody detects EV71 VP3 protein by immunofluorescent analysis. Sample: RD cells infected with EV71 were fixed in 4% paraformaldehyde. Green: EV71 VP3 protein stained by EV71 VP3 antibody (GTX132341) diluted at 1:100. Blue: DAPI staining. Scale bar = 500 μm.

GTX132341 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EV71 VP3 antibody (GTX132341) diluted at 1:1000.

REFERENCE

Application Reference

Yu J et al. Virulence 2022; 13 (1) : 241-257 Regulation of host factor γ-H2AX level and location by enterovirus A71 for viral replication.

Application : WB

Su YS et al. Front Microbiol 2020; 11 : 1656 The Heat Shock Protein 70 Family of Chaperones Regulates All Phases of the Enterovirus A71 Life Cycle.

Application : WB

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