GeneTex

  • Country / Location Selection

United States (US)

Japanese encephalitis virus Envelope antibody

Anti-Japanese encephalitis virus Envelope antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125867
Anti-Japanese encephalitis virus Envelope antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125867
Anti-Japanese encephalitis virus Envelope antibody used in Western Blot (WB). GTX125867
Anti-Japanese encephalitis virus Envelope antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX125867
Anti-Japanese encephalitis virus Envelope antibody used in Western Blot (WB). GTX125867
Anti-Japanese encephalitis virus Envelope antibody used in Western Blot (WB). GTX125867

Cat. No. GTX125867

Reference 15
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-P (cell pellet)

Reactivity

 Japanese encephalitis virus
Package
100 μl ($399),
25 μl ($169)
See all Japanese encephalitis virus Envelope products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

Japanese encephalitis virus infected BHK-21 cells

Product Note

This antibody is specific for JEV Envelope protein, and it does not cross-react with Zika, DENV-1, DENV-2, DENV-3, and DENV-4 Envelope protein.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Japanese encephalitis virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Envelope protein (JEV). (Japanese Encephalitis Virus strain Jaoars982) The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172365

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Japanese encephalitis Virus Envelope , JEV Envelope

Cellular Localization

Virion membrane; Multi-pass membrane protein. Host endoplasmic reticulum membrane; Multi-pass membrane protein.

Research Area

DATA IMAGES

Anti-Japanese encephalitis virus Envelope antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125867

GTX125867 ICC/IF Image

Envelope protein (Japanese encephalitis virus) antibody detects envelope protein (Japanese encephalitis virus) protein by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis viruswere fixed in MeOH.
Green: envelope protein (Japanese encephalitis virus) protein stained by Envelope protein (Japanese encephalitis virus) antibody (GTX125867) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Japanese encephalitis virus Envelope antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125867

GTX125867 ICC/IF Image

Envelope protein (Japanese encephalitis virus) antibody detects envelope protein (Japanese encephalitis virus) protein by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis viruswere fixed in paraformaldehyde.
Green: envelope protein (Japanese encephalitis virus) protein stained by Envelope protein (Japanese encephalitis virus) antibody (GTX125867) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Japanese encephalitis virus Envelope antibody used in Western Blot (WB). GTX125867

GTX125867 WB Image

envelope protein (Japanese encephalitis virus) antibody detects envelope protein (Japanese encephalitis virus) protein by western blot analysis.
A. 10 μg BHK-21 whole cell extract
B. 10 μg whole cell extract of Japanese encephalitis virus infected BHK-21 cells
C. 10 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
10% SDS-PAGE
envelope protein (Japanese encephalitis virus) antibody (GTX125867) dilution: 1:20000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Japanese encephalitis virus Envelope antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX125867

GTX125867 IHC-P (cell pellet) Image

Envelope protein (JEV) antibody detects Envelope protein (JEV) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded japanese encephalitis virus BHK-21.
Green: Envelope protein (JEV) stained by Envelope protein (JEV) antibody (GTX125867) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Japanese encephalitis virus Envelope antibody used in Western Blot (WB). GTX125867

GTX125867 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with Japanese encephalitis virus Envelope antibody (GTX125867) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Japanese encephalitis virus Envelope antibody used in Western Blot (WB). GTX125867

GTX125867 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Japanese encephalitis virus Envelope antibody (GTX125867) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Fujiwara Y et al. RNA Biol 2024; 21 (1) : 1-9 Expression of RNautophagy/DNautophagy-related genes is regulated under control of an innate immune receptor.
Z Chen et al. Virol J 2023; 20 (1) : 280 Porcine promyelocytic leukemia protein isoforms suppress Japanese encephalitis virus replication in PK15 cells
Ishida K et al. PLoS Pathog 2023; 19 (10) : e1011681 N-linked glycosylation of flavivirus E protein contributes to viral particle formation.
K Tabata et al. Appl Microbiol Biotechnol 2023; Development of flavivirus subviral particles with low cross-reactivity by mutations of a distinct antigenic domain
Liang Z et al. Viruses 2023; 15 (7) Interaction between hTIM-1 and Envelope Protein Is Important for JEV Infection.
Show More +
Submit a Reference

REVIEW

Japanese encephalitis virus Envelope antibody Cat. No. GTX125867

Rating
( Average 4 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 1 users reviews)
Anti-Japanese encephalitis virus Envelope antibody used in IHC (Paraffin sections) (IHC-P). GTX125867
Date : Anonymous submitted on 26-Aug-2014
Score :
Application Tested : IHC-P
Sample Species : JEV
Sample : Japanese encephalitis virus infected mouse brain
Blocking : Normal Goat Serum Blocking in TBS, 25°C, 0.3Hr
Fixation : 10% Formalin
Permeabilization /Antigen retrieval : TE buffer
Primary Antibody : 1:2000, 4°C, 16Hr
Submit a Review
Application Reference
Fujiwara Y et al. RNA Biol 2024; 21 (1):1-9 Expression of RNautophagy/DNautophagy-related genes is regulated under control of an innate immune receptor.
Z Chen et al. Virol J 2023; 20 (1):280 Porcine promyelocytic leukemia protein isoforms suppress Japanese encephalitis virus replication in PK15 cells
Ishida K et al. PLoS Pathog 2023; 19 (10):e1011681 N-linked glycosylation of flavivirus E protein contributes to viral particle formation.
K Tabata et al. Appl Microbiol Biotechnol 2023; Development of flavivirus subviral particles with low cross-reactivity by mutations of a distinct antigenic domain
Liang Z et al. Viruses 2023; 15 (7) Interaction between hTIM-1 and Envelope Protein Is Important for JEV Infection.
Yang H et al. J Virol 2023; 97 (5):e0165822 Peripheral Nerve Injury Induced by Japanese Encephalitis Virus in C57BL/6 Mouse.
Xing J et al. Microbiol Spectr 2023;:e0318622 USP1-Associated Factor 1 Modulates Japanese Encephalitis Virus Replication by Governing Autophagy and Interferon-Stimulated Genes
Application : WB
Wang X et al. PLoS Negl Trop Dis 2022; 16 (11):e0010961 A mouse model of peripheral nerve injury induced by Japanese encephalitis virus.
Application : WB
Xiaochun Ma et al. Viruses 2022; 14 (4) Construction of a Recombinant Japanese Encephalitis Virus with a Hemagglutinin-Tagged NS2A: A Model for an Analysis of Biological Characteristics and Functions of NS2A during Viral Infection.
Keisuke Tabata et al. J Virol 2021; 95 (15):e0223420 Endoplasmic Reticulum-Associated Degradation Controls Virus Protein Homeostasis, Which Is Required for Flavivirus Propagation.
Application : WB
Nath B et al. Vaccine 2020; 38 (7):1860-1868 Evaluation of Japanese encephalitis virus E and NS1 proteins immunogenicity using a recombinant Newcastle disease virus in mice.
Garg H et al. Sci Rep 2020; 10 (1):4017 Virus Like Particles (VLP) as multivalent vaccine candidate against Chikungunya, Japanese Encephalitis, Yellow Fever and Zika Virus.
Application : WB
Fu TL et al. J Neuropathol Exp Neurol 2019; 78 (12):1160-1170 Japanese Encephalitis Virus Infects the Thalamus Early Followed by Sensory-Associated Cortex and Other Parts of the Central and Peripheral Nervous Systems.
Application : IHC-P
Li PC et al. ACS Infect Dis 2019; (Epub) Small molecules targeting the flavivirus E protein with broad-spectrum activity and antiviral efficacy in vivo.
Lu CY et al. Viruses 2017; 9 (4) Single-Round Infectious Particle Antiviral Screening Assays for the Japanese Encephalitis Virus.
Application : ICC/IF
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169