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EpCAM antibody [2H9]

Overexpression
Anti-EpCAM antibody [2H9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84573
Anti-EpCAM antibody [2H9] used in Western Blot (WB). GTX84573
Anti-EpCAM antibody [2H9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84573
Anti-EpCAM antibody [2H9] used in Western Blot (WB). GTX84573
Anti-EpCAM antibody [2H9] used in Western Blot (WB). GTX84573

Cat. No. GTX84573

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2H9

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($519)
See all EpCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:500
ICC/IF 1:100
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 24-265 of human EpCAM

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1

Background

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

Database

Research Area

DATA IMAGES

Anti-EpCAM antibody [2H9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84573

GTX84573 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX84573 EpCAM antibody [2H9].

Anti-EpCAM antibody [2H9] used in Western Blot (WB). GTX84573

GTX84573 WB Image

WB analysis of HT29 cell lysate using GTX84573 EpCAM antibody [2H9].
Loading : 10 ug per lane
Dilution : 1:200

Anti-EpCAM antibody [2H9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84573

GTX84573 ICC/IF Image

ICC/IF analysis of HeLa cells transiently transfected with EpCAM plasmid using GTX84573 EpCAM antibody [2H9].

Anti-EpCAM antibody [2H9] used in Western Blot (WB). GTX84573

GTX84573 WB Image

WB analysis of various samples using GTX84573 EpCAM antibody [2H9].
Loading : 10 ug per lane
Dilution : 1:200

Anti-EpCAM antibody [2H9] used in Western Blot (WB). GTX84573

GTX84573 WB Image

Overexpression

WB analysis of HEK293T cells transfected with EpCAM plasmid (Right) or empty vector (Left) for 48 hrs using GTX84573 EpCAM antibody [2H9].
Loading : 5 ug per lane

REFERENCE

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REVIEW

There are currently no reviews for EpCAM antibody [2H9] (GTX84573). Be the first to share your experience with this product.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519