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EpCAM antibody [HEA125]

Cat. No. GTX76570

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HEA125

Isotype

 IgG1

Application

 ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human
Package
100 μg ($1059)
See all EpCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P 1/20
IHC-Fr 1/20
FACS Neat

Note :

IHC-P
This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

FACS
Use 5μl of the suggested working dilution to label 1006 cells or 100μl celll suspension.
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

HT29 carcinoma cell line.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_377419

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

REFERENCE

Application Reference

Torsello B et al. J Cell Sci 2016; (Epub) Arg/Abl2 tyrosine kinase modulates TGF-β1 production in human renal tubular cells under high glucose condition.
Application : ICC/IF
Reactivity : Human

Clone Reference

Yanamoto S et al. Oral Oncol 2007; 43 (9):869-77 Clinicopathologic significance of EpCAM expression in squamous cell carcinoma of the tongue and its possibility as a potential target for tongue cancer gene therapy.
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 1059