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EphB1 antibody [5F10A4]

Anti-EphB1 antibody [5F10A4] used in IHC (Paraffin sections) (IHC-P). GTX83049
Anti-EphB1 antibody [5F10A4] used in Western Blot (WB). GTX83049

Cat. No. GTX83049

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5F10A4

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($409)
See all EphB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Product Note

It is only specific to EPHB1

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of EphB1 (aa19-133) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EPH receptor B1 , ELK , EPHT2 , Hek6 , NET

Cellular Localization

Cell membrane,Cell projection, dendrite

Background

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EphB1 antibody [5F10A4] used in IHC (Paraffin sections) (IHC-P). GTX83049

GTX83049 IHC-P Image

IHC-P analysis of human lung cancer (left) and colon cancer (right) using GTX83049 EphB1 antibody [5F10A4].

Anti-EphB1 antibody [5F10A4] used in Western Blot (WB). GTX83049

GTX83049 WB Image

WB analysis of MDA-MB-468 (1), MDA-MB-453 (2), MCF-7 (3), T47D (4) and SKBR-3 (5) cell lysate using GTX83049 EphB1 antibody [5F10A4].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409