*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HUVEC , HeLa , C8D30 , NIH-3T3 , C2C12 , Rat2 , HUVEC (5 ng/ml VEGF treatment for 10 min) , HepG2 (200 ng/ml EGF treatment for 30 min)
Bovine, Chicken(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1.63 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide corresponding to residues around human FAK (phospho Tyr397). The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Protein Tyrosine Kinase 2,Fadk,Fak,Fak1,Frnk,Ppp1R71,P125Fak,Pp125Fak,Ptk2
Cell junction , focal adhesion , Cell membrane; Peripheral membrane protein; Cytoplasmic side
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. [provided by RefSeq]