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FAM59A antibody

Anti-FAM59A antibody used in IHC (Paraffin sections) (IHC-P). GTX85231
Anti-FAM59A antibody used in IHC (Paraffin sections) (IHC-P). GTX85231
Anti-FAM59A antibody used in Western Blot (WB). GTX85231

Cat No. GTX85231

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
Package
100 μg ($339)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Rat Liver Tissue Lysate(GTX27943)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

FAM59A antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FAM59A.The immunogen is located within amino acids 630 - 680 of FAM59A.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Grb2 Associated Regulator Of Mapk1 Subtype 1,C18Orf11,Fam59A,Garem,Gm944,Garem1

Background

FAM59A, also known as GAREM, was initially identified through a mass spectroscoptic method that measures different phosphotyrosine states of proteins in response to different cell stimuli. FAM59A is a downstream molecule in the EGF signaling pathway that is tyrosine-phosphorylated, and this phosphorylation is needed for the binding of Grb2, an adaptor protein crucial to the transduction of growth signals from the plasma membrane to the nucleus. ERK activation in response to EGF stimulation is regulated by FAM59A in COS-7 and HeLa cells, and the overexpression of FAM59A stimulated cell proliferation and colony formation in soft agar, suggesting that FAM59A might be a critical protein with roles in ligand-mediated signaling pathway of the EGF receptor and the tumorigenesis of cells.

Database

DATA IMAGES
Anti-FAM59A antibody used in IHC (Paraffin sections) (IHC-P). GTX85231

GTX85231 IHC-P Image

IHC-P analysis of human liver tissue using GTX85231 FAM59A antibody.
Working concentration : 20 μg/ml

Anti-FAM59A antibody used in IHC (Paraffin sections) (IHC-P). GTX85231

GTX85231 IHC-P Image

IHC-P analysis of human brain tissue using GTX85231 FAM59A antibody.
Working concentration : 5 μg/ml

Anti-FAM59A antibody used in Western Blot (WB). GTX85231

GTX85231 WB Image

WB analysis of rat liver tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX85231 FAM59A antibody.
Working concentration : 1 μg/ml

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Package List Price ($)
$ 339