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FBXL3 antibody

Anti-FBXL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110755
Anti-FBXL3 antibody used in Western Blot (WB). GTX110755
Anti-FBXL3 antibody used in Western Blot (WB). GTX110755
Anti-FBXL3 antibody used in Western Blot (WB). GTX110755
Anti-FBXL3 antibody used in Western Blot (WB). GTX110755

Cat. No. GTX110755

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

C2C12 , Molt-4

Predict Reactivity

Rat, Sheep, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FBXL3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950264

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

F-box and leucine rich repeat protein 3 , FBL3 , FBL3A , FBXL3A

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats and is localized in the nucleus. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FBXL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110755

GTX110755 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using FBXL3(GTX110755) antibody at 1:200 dilution.

Anti-FBXL3 antibody used in Western Blot (WB). GTX110755

GTX110755 WB Image

FBXL3 antibody detects FBXL3 protein by Western blot analysis.
A. 30 μg C2C12 whole cell lysate/extract
10 % SDS-PAGE
FBXL3 antibody (GTX110755) dilution: 1:500

Anti-FBXL3 antibody used in Western Blot (WB). GTX110755

GTX110755 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
10% SDS PAGE
GTX110755 diluted at 1:1000

Anti-FBXL3 antibody used in Western Blot (WB). GTX110755

GTX110755 WB Image

The data was published in the journal Nat Commun in 2017.PMID: 28090088

Anti-FBXL3 antibody used in Western Blot (WB). GTX110755

GTX110755 WB Image

The data was published in the journal Nat Commun in 2017.PMID: 28090088

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169