GeneTex
United States (US)

FGF1 recombinant protein

Cat No. GTX65338

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Mouse
Species Mouse
APPLICATION

Application Note

The ED50 as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of heparin is 0.5 ng/ml, corresponding to a specific activity of >2 x10^5 units/mg.

Calculated MW

15.9 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Buffer

1 mg/ml solution of 5 mM NaP, pH 7.5 + 75 mM NaCl.

Storage

Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4ºC for 1 week or –20ºC for future use. Repeated freeze/thaw cycles will result in some loss of activity.

Antigen Species

Mouse

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ECGFA, HBGF1, ECGF-beta, FGF-a, FGFIBP, HBGF-1, ECGF, FGFa, FIBP-1, FIBP1, FIBP, FGF1, GLIO703, Fibroblast Growth Factor, ECGFbeta, FGF a, HBGF 1, FIBP 1, ECGF beta

Background

FGF-1 is an acidic heparin binding growth factor that stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. Recombinant murine FGF-1 is a 15.9 kDa protein containing 141 amino acid residues.

Research Area