FGF1 recombinant protein

Cat No. GTX65339

Application	ELISA, Functional Assay, Apuri, Blocking
Reactivity	Mouse
Species	Mouse

See all FGF1 products

Datasheet

APPLICATION

Application Note

The ED50 as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of heparin is 0.5 ng/ml, corresponding to a specific activity of >2 x10^5units/mg.

Calculated MW

15.9 kDa. ( Note )

PROPERTIES

Form

Lyophilized powder

Buffer

1 mg/ml solution of 5 mM NaP, pH 7.5 + 75 mM NaCl.

Storage

Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4ºC for 1 week or –20ºC for future use. Repeated freeze/thaw cycles will result in some loss of activity.

Antigen Species

Mouse

Expression System

E. coli

Purification

Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Fibroblast Growth Factor 1,Dffrx,Fam,Fgf-1,Fgfa,Fgf1

Background

FGF-1 is an acidic heparin binding growth factor that stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. Recombinant murine FGF-1 is a 15.9 kDa protein containing 141 amino acid residues.