The ED50 determined by the dose-dependant proliferation of BALB/3T3 cells is <0.2 ng/ml, corresponding to a specific activity of 5x10^6 IU/mg.
1 mg/ml solution after extensive dialysis against 20 mM phosphate buffer, pH 7.4 and 130 mM NaCl.
Reconstitute in sterile water to 0.1–1 mg /ml. The solution can then further diluted to other aqueous solutions. Stored at -20ºC for future use.
Purity was assessed by SDS-PAGE (≥97%) and by HPLC (≥97%).
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
FGFb, HBGH-2, Prostatropin, HBGF2, HBGF-2, FGF2, FGF-2, HBGH2, Fibroblast Growth Factor, FGF-b, FGF 2, FGF b, HBGH 2, HBGF 2
FGF-2 is a basic heparin binding growth factor that stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. The recombinant rat FGF-2 is a single, non-glycosylated chain containing 145 amino acids and having a molecular weight of 16.3 kDa.