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Factor B antibody [N2C2], Internal

Orthogonal
Anti-Factor B antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103570
Anti-Factor B antibody [N2C2], Internal used in Western Blot (WB). GTX103570
Anti-Factor B antibody [N2C2], Internal used in Western Blot (WB). GTX103570
Anti-Factor B antibody [N2C2], Internal used in Western Blot (WB). GTX103570

Cat. No. GTX103570

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Factor B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Factor B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164587

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

complement factor B , AHUS4 , ARMD14 , BF , BFD , CFAB , CFBD , FB , FBI12 , GBG , H2-Bf , PBF2

Cellular Localization

Secreted

Background

This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Factor B antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103570

GTX103570 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Factor B(GTX103570) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Factor B antibody [N2C2], Internal used in Western Blot (WB). GTX103570

GTX103570 WB Image

Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Factor B antibody [N2C2], Internal (GTX103570) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Factor B antibody [N2C2], Internal used in Western Blot (WB). GTX103570

GTX103570 WB Image

Rat plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Factor B antibody [N2C2], Internal (GTX103570) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Factor B antibody [N2C2], Internal used in Western Blot (WB). GTX103570

GTX103570 WB Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2020.PMID: 32054021

REFERENCE

Application Reference

Zhu Y et al. Int J Occup Med Environ Health 2021; Evaluation of differential serum expression of three factors and pulmonary function in patients with silicosis.
Application : WB
Reactivity : Human
Zhu Y et al. Int J Mol Sci 2020; 21 (4) Protein Expression Profile in Rat Silicosis Model Reveals Upregulation of PTPN2 and Its Inhibitory Effect on Epithelial-Mesenchymal Transition by Dephosphorylation of STAT3.
Application : WB
Reactivity : Rat
Yang C.S. et al. Mol Biosyst 2017; (Epub) Proteomic Analysis of Prognostic Plasma Biomarkers in Peripheral Arterial Occlusive Disease.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169