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Fatty Acid Synthase antibody

KO/KD
Anti-Fatty Acid Synthase antibody used in IHC (Paraffin sections) (IHC-P). GTX35200
Anti-Fatty Acid Synthase antibody used in IHC (Paraffin sections) (IHC-P). GTX35200
Anti-Fatty Acid Synthase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35200
Anti-Fatty Acid Synthase antibody used in Western Blot (WB). GTX35200
Anti-Fatty Acid Synthase antibody used in Western Blot (WB). GTX35200
Anti-Fatty Acid Synthase antibody used in Immunoprecipitation (IP). GTX35200

Cat. No. GTX35200

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399)
See all Fatty Acid Synthase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:100
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

273 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human FASN (NP_004095.4).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fatty acid synthase , FAS , OA-519 , SDR27X1

Cellular Localization

Cytoplasm

Background

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Fatty Acid Synthase antibody used in IHC (Paraffin sections) (IHC-P). GTX35200

GTX35200 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX35200 Fatty Acid Synthase antibody.
Dilution : 1:100

Anti-Fatty Acid Synthase antibody used in IHC (Paraffin sections) (IHC-P). GTX35200

GTX35200 IHC-P Image

IHC-P analysis of human colon tissue using GTX35200 Fatty Acid Synthase antibody.
Dilution : 1:100

Anti-Fatty Acid Synthase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35200

GTX35200 ICC/IF Image

ICC/IF analysis of A549 cells using GTX35200 Fatty Acid Synthase antibody.
Blue : DAPI

Anti-Fatty Acid Synthase antibody used in Western Blot (WB). GTX35200

GTX35200 WB Image

WB analysis of H460 cell lysate using GTX35200 Fatty Acid Synthase antibody.
Dilution : 1:200
Loading : 25μg per lane

Anti-Fatty Acid Synthase antibody used in Western Blot (WB). GTX35200

GTX35200 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX35200 Fatty Acid Synthase antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Fatty Acid Synthase antibody used in Immunoprecipitation (IP). GTX35200

GTX35200 IP Image

IP analysis of H460 cell lysate using GTX35200 Fatty Acid Synthase antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399