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Fibronectin antibody [2755-8.]

Anti-Fibronectin antibody [2755-8.] used in IHC (Paraffin sections) (IHC-P). GTX34729

Cat No. GTX34729

Host Mouse
Clonality Monoclonal
Clone Name 2755-8.
Isotype IgG1
Application ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

263 kDa. ( Note )

Specificity/Sensitivity

Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that Myofibroblasts phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytrenā€™s disease.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

T-cell lymphoma biopsy

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fibronectin 1,Cig,Ed-B,Finc,Fn,Fnz,Gfnd,Gfnd2,Lets,Msf,Smdcf,Fn1

Background

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Fibronectin antibody [2755-8.] used in IHC (Paraffin sections) (IHC-P). GTX34729

GTX34729 IHC-P Image

Formalin-fixed, paraffin-embedded human Pancreatic Adenocarcinoma stained with Fibronectin Monoclonal Antibody (2755-8).

Package List Price ($)
$ 329