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Filamin A antibody [C2], C-term

Anti-Filamin A antibody [C2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112939
Anti-Filamin A antibody [C2], C-term used in Western Blot (WB). GTX112939
Anti-Filamin A antibody [C2], C-term used in Western Blot (WB). GTX112939
Anti-Filamin A antibody [C2], C-term used in IHC (Paraffin sections) (IHC-P). GTX112939
Anti-Filamin A antibody [C2], C-term used in Western Blot (WB). GTX112939

Cat. No. GTX112939

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Filamin A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

281 kDa. ( Note )

Positive Control

Mouse kidney , H1299 , HepG2 , PC-12 , Rat2

Predict Reactivity

Rabbit, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Filamin A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036932

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

filamin A , ABP-280 , ABPX , CSBS , CVD1 , FGS2 , FLN , FLN-A , FLN1 , FMD , MNS , NHBP , OPD , OPD1 , OPD2 , XLVD , XMVD

Cellular Localization

Cytoplasm , cell cortex , cytoskeleton

Background

The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.

Database

Research Area

DATA IMAGES

Anti-Filamin A antibody [C2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112939

GTX112939 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Filamin A alpha 2(GTX112939) antibody at 1:200 dilution.

Anti-Filamin A antibody [C2], C-term used in Western Blot (WB). GTX112939

GTX112939 WB Image

Filamin A antibody detects Filamin A protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
5 % SDS-PAGE
Filamin A antibody (GTX112939) dilution: 1:1000

Anti-Filamin A antibody [C2], C-term used in Western Blot (WB). GTX112939

GTX112939 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hep G2 (GTX27900)
5% SDS PAGE
GTX112939 diluted at 1:1000

Anti-Filamin A antibody [C2], C-term used in IHC (Paraffin sections) (IHC-P). GTX112939

GTX112939 IHC-P Image

Filamin A antibody [C2], C-term detects Filamin A protein at cytosol on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Filamin A antibody [C2], C-term (GTX112939) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Filamin A antibody [C2], C-term used in Western Blot (WB). GTX112939

GTX112939 WB Image

Sample (50 ug of whole cell lysate)
A: mouse kidney
5% SDS PAGE
GTX112939 diluted at 1:1000

REFERENCE

Application Reference

Salim C et al. J Orofac Orthop 2021; Role of chaperone-assisted selective autophagy (CASA) in mechanical stress protection of periodontal ligament cells.
Application : WB
Reactivity : Human
Yang HH et al. Proteomics Clin Appl 2016; (Epub) the putative involvement of actin binding proteins and cytoskeleton proteins in pathological mechanisms of * cystitis - revealed by a prospective pilot study using proteomic approaches.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169