GeneTex
United States (US)

Flt3 Ligand Recombinant protein

flt-3-Ligand-recombinant-protein_WB_GTX65342-1_18121410_144.jpg

Cat No. GTX65342

Application WB, ELISA, Functional Assay, Apuri, Blocking
Reactivity Mouse
Species Mouse
APPLICATION

Application Note

Protein's bioactivity determined by its ability to generate CD11c+ splenic dendritic cells in vivo in C57B1/6 mice (female, approx. 12 weeks old). To obtain the desired affect, mice were treated with approximately 25 ug of flt3-ligand once daily for 9 consecutive days via intraperitoneal injection.

Calculated MW

16.4 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Storage

Reconstitute in water to a concentration of 0.1 – 1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4ºC for 1 week or –20ºC for future use.

Antigen Species

Mouse

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fms-related tyrosine kinase 3 ligand

Background

Flt3-Ligand belongs to a small group of growth factors that regulate proliferation of early hematopoietic cells. Multiple isoforms of Flt3-Ligand have been identified. The predominant form is the transmembrane which is biologically active on the cell surface. The proteolytically cleaved transmembrane isoform generates a soluble form, which is also biologically active. Flt3-Ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3-Ligand alone cannot stimulate proliferation, but synergizes well with other CSFs and interleukins to induce growth and differentiation. Recombinant murine flt3-Ligand is a 16.4 kDa protein containing 144 amino acid residues.
DATA IMAGES
flt-3-Ligand-recombinant-protein_WB_GTX65342-1_18121410_144.jpg

GTX65342 WB Image

Lane 1: Marker
Lane 2: ~10 µg rm FLT3L

Package List Price ($)
$ 249