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Frizzled 6 antibody

Anti-Frizzled 6 antibody used in Western Blot (WB). GTX64757

Cat. No. GTX64757

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse, Rat
Package
100 μl ($399)
See all Frizzled 6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:2000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 19-201 of human FZD6 (NP_003497.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2888607

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

frizzled class receptor 6 , FZ-6 , FZ6 , HFZ6 , NDNC10

Cellular Localization

Membrane,Cell membrane

Background

This gene represents a member of the 'frizzled' gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants, some of which do not encode a protein with a predicted signal peptide.[provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-Frizzled 6 antibody used in Western Blot (WB). GTX64757

GTX64757 WB Image

WB analysis of various sample lysates using GTX64757 Frizzled 6 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Johansson J et al. Cell Stem Cell 2019; 24 (4) : 592-607.e7 RAL GTPases Drive Intestinal Stem Cell Function and Regeneration through Internalization of WNT Signalosomes.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399