GeneTex
United States (US)

G protein alpha Inhibitor 3 antibody

KO/KD
GTX35215_20180822_WB_1_18121410_256.jpg
GTX35215_20180822_WB_18121410_235.jpg
GTX35215_20180822_IP_18121410_102.jpg

Cat No. GTX35215

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

41 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI3 (NP_006487.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ARCND1 Antibody , 87U6 Antibody , GNAI3 Antibody , guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 Antibody

Background

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012]
Show More +
DATA IMAGES
GTX35215_20180822_WB_1_18121410_256.jpg

GTX35215 WB Image

KO/KD

WB analysis of wild type and G protein alpha Inhibitor 3 knockout cell lysate using GTX35215 G protein alpha Inhibitor 3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX35215_20180822_WB_18121410_235.jpg

GTX35215 WB Image

KO/KD

WB analysis of various samples using GTX35215 G protein alpha Inhibitor 3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX35215_20180822_IP_18121410_102.jpg

GTX35215 IP Image

KO/KD

IP analysis of MCF7 cell lysate using GTX35215 G protein alpha Inhibitor 3 antibody.
IP reaction : 3μg antibody / 200μg cell lysate
Dilution : 1:1000

Package List Price ($)
$ 329