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GAB1 antibody

Anti-GAB1 antibody used in Western Blot (WB). GTX111253
Anti-GAB1 antibody used in Western Blot (WB). GTX111253
Anti-GAB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111253

Cat. No. GTX111253

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GAB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

HCT116 , MCF-7 , JC , BCL-1

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11178858

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GRB2 associated binding protein 1 , DFNB26

Background

The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GAB1 antibody used in Western Blot (WB). GTX111253

GTX111253 WB Image

Sample (30 μg of whole cell lysate)
A: HCT116
B: MCF-7
7.5% SDS PAGE
GTX111253 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAB1 antibody used in Western Blot (WB). GTX111253

GTX111253 WB Image

Sample (30 μg of whole cell lysate)
A: JC
B: BCL-1
7.5% SDS PAGE
GTX111253 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111253

GTX111253 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using GAB1(GTX111253) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Li Y et al. Cell Death Dis 2023; 14 (8) : 494 Smurf1 and Smurf2 mediated polyubiquitination and degradation of RNF220 suppresses Shh-group medulloblastoma.
Hamam SM et al. Childs Nerv Syst 2023; 39 (7) : 1869-1880 Prognostic value of microRNA-125a expression status in molecular groups of pediatric medulloblastoma.
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REVIEW

GAB1 antibody Cat. No. GTX111253

Rating
( Average 3 based on 1 users reviews)
Application
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-GAB1 antibody used in Western Blot (WB). GTX111253
Date : Anonymous submitted on 02-Dec-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : C2C12 cells
Amount used : 20μg
Blocking : 5% skim milk in 0.05% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169