GeneTex
United States (US)

GAD65 antibody [N-GAD65]

GTX30009_3742_WB_18121410_608.jpg

Cat No. GTX30009

Host Mouse
Clonality Monoclonal
Clone Name N-GAD65
Isotype IgG1
Application WB, IHC-P, IP, RIA
Reactivity Human, Rat, Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/100-1/1,000
IHC-P Assay dependent
IP Assay dependent
RIA Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Specificity/Sensitivity

Clone N-GAD65 recognizes GAD65 (also known as GAD2) but does not recognize GAD67 (also known as GAD1).
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Keyhole Limpet Haemocyanin (KLH) conjugated synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-terminal region of human GAD65.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutamate Decarboxylase 2,Gad65,Gad2

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES
GTX30009_3742_WB_18121410_608.jpg

GTX30009 WB Image

WB analysis of human brain cortex tissue lysate using GTX30009 GAD65 antibody [N-GAD65] followed by Goat anti Mouse IgG H+L:DyLight®800 secondary antibody

Package List Price ($)
$ 329