GeneTex
United States (US)

GADD34 antibody

GADD34-antibody-GTX29869-WB-1_18121410_162.jpg
GADD34-antibody_WB_GTX29869-1_18121410_533.jpg

Cat No. GTX29869

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

Western Blot - Approx 80-90 kDa band seen in U937 Lysate. Recommended for use at 0.5-2 μg/ml.Please note that although the predicted size of GADD34 is approximately 75kDa according to NP_055145, Adler et al., [Mol. Cell. Biol., Oct 1999; 19: 7050 - 7060] observe GADD34 migrating at approx 90kDa on SDS-PAGE.

Calculated MW

73 kDa. ( Note )

Positive Control

U937 lysate
PROPERTIES

Form

Liquid

Buffer

Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence AAALDLSGRRG, from C-Terminus of the protein sequence according to NP_055145

Purification

IgG fraction
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Phosphatase 1 Regulatory Subunit 15A,Gadd34,Ppp1R15A

Background

A protein phosphatase whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents.

Database

Research Area

DATA IMAGES
GADD34-antibody-GTX29869-WB-1_18121410_162.jpg

GTX29869 WB Image

Staining (0.5g/ml) of U937 lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

GADD34-antibody_WB_GTX29869-1_18121410_533.jpg

GTX29869 WB Image

Western blot analysis of GADD34 in HEPG2 cell lysate (35µg protein in RIPA buffer) using GTX29869 at 0.1 ug/ml.

Package List Price ($)
$ 319