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GADD45A antibody

KO/KD Overexpression
Anti-GADD45A antibody used in Western Blot (WB). GTX54090
Anti-GADD45A antibody used in Western Blot (WB). GTX54090

Cat. No. GTX54090

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse
Package
100 μl ($399)
See all GADD45A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human GADD45A (NP_001915.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887840

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

growth arrest and DNA damage inducible alpha , DDIT1 , GADD45

Cellular Localization

Nucleus

Background

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-GADD45A antibody used in Western Blot (WB). GTX54090

GTX54090 WB Image

WB analysis of mouse liver tissue lysate using GTX54090 GADD45A antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-GADD45A antibody used in Western Blot (WB). GTX54090

GTX54090 WB Image

KO/KD Overexpression

The data was published in the 2020 in Cell Death Dis. PMID: 32719383

REFERENCE

Application Reference

You W et al. Cell Death Dis 2020; 11 (7) : 585 GADD45α drives brown adipose tissue formation through upregulating PPARγ in mice.
Application : WB
Reactivity : Mouse
Liu SY et al. Br J Pharmacol 2019; 176 (11) : 1793-1810 Protein inhibitor of activated STAT1 Ser503 phosphorylation-mediated Elk-1 SUMOylation promotes neuronal survival in APP/PS1 mice.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399