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GAP43 antibody

Anti-GAP43 antibody used in Western Blot (WB). GTX127937
Anti-GAP43 antibody used in IHC (Frozen sections) (IHC-Fr). GTX127937
Anti-GAP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127937
Anti-GAP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX127937
Anti-GAP43 antibody used in Western Blot (WB). GTX127937
Anti-GAP43 antibody used in Western Blot (WB). GTX127937
Anti-GAP43 antibody used in Western Blot (WB). GTX127937
Anti-GAP43 antibody used in Western Blot (WB). GTX127937

Cat. No. GTX127937

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GAP43 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:30000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

mouse hippocampus , rat hippocampus , mouse fetal brain , mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of rat GAP43. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885659

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

growth associated protein 43 , Basp2

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell projection , growth cone membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , synapse

Background

may play a role in neurite outgrowth and nerve regeneration [RGD]

Database

Research Area

DATA IMAGES

Anti-GAP43 antibody used in Western Blot (WB). GTX127937

GTX127937 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody (GTX127937) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAP43 antibody used in IHC (Frozen sections) (IHC-Fr). GTX127937

GTX127937 IHC-Fr Image

GAP43 antibody detects GAP43 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: GAP43 protein stained by GAP43 antibody (GTX127937) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Anti-GAP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127937

GTX127937 ICC/IF Image

GAP43 antibody detects GAP43 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAP43 protein stained by GAP43 antibody (GTX127937) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-GAP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX127937

GTX127937 IHC-P Image

GAP43 antibody detects Gap43 protein at on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
GAP43 antibody (GTX127937) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GAP43 antibody used in Western Blot (WB). GTX127937

GTX127937 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody (GTX127937) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAP43 antibody used in Western Blot (WB). GTX127937

GTX127937 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody (GTX127937) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAP43 antibody used in Western Blot (WB). GTX127937

GTX127937 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody (GTX127937) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAP43 antibody used in Western Blot (WB). GTX127937

GTX127937 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody (GTX127937) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Li P et al. Front Cardiovasc Med 2022; 9 : 1019435 lncRNA LOC100911717-targeting GAP43-mediated sympathetic remodeling after myocardial infarction in rats.
Application : WB, IHC-P
Reactivity : Rat
Otsuka T et al. Stem Cells Dev 2021; Comparisons of Neurotrophic Effects of Mesenchymal Stem Cells Derived from Different Tissues on Chronic Spinal Cord Injury Rats.
Liu C et al. Bioact Mater 2021; 6 (11) : 3892-3903 Self-curling electroconductive nerve dressing for enhancing peripheral nerve regeneration in diabetic rats.
Application : ICC/IF
Reactivity : Rat
Dellarole A et al. Brain Behav Immun 2014; 41 : 65-81 Neuropathic pain-induced depressive-like behavior and hippocampal neurogenesis and plasticity are dependent on TNFR1 signaling.
Application : WB
Reactivity : Mouse
Dellarole A et al. Brain Behav Immun 2014; (Epub) Neuropathic pain-induced depressive-like behavior and hippocampal neurogenesis and plasticity are dependent on TNFR1 signaling.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169