GeneTex
United States (US)

GAPDH recombinant protein

Cat No. GTX65100

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Calculated MW

36.0 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

20 mM Tris pH-8, 1 mM EDTA, 1 mM DTT, and 20% glycerol.

Storage

Store at -20ºC. Product is stable for at least 6-12 months.

Antigen Species

Human

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥95%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glyceraldehyde 3phosphate dehydrogenase, Glyceraldehyde 3-phosphate dehydrogenase, Glyceraldehyde3phosphate dehydrogenase, GD3, GAPDH

Background

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a catalytic enzyme normally known to play a role in glycolysis. GAPDH exists as a tetramer composed of 36-kDa subunits and has a range of intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, GAPDH has function in intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. GAPDH catalyzes a vital energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Recombinant human GAPDH produced in E.Coli is a single, non-glycosylated polypeptide chain containing 335 amino acids and having a molecular mass of 36 kDa. The GAPDH is purified by proprietary chromatographic techniques.

Research Area

Package List Price ($)
$ 329