APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , MCF-7 , mouse testis
Predict Reactivity
Rat, Rabbit, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.91 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human GAPDHS. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10625106
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glyceraldehyde-3-phosphate dehydrogenase, spermatogenic , GAPD2 , GAPDH-2 , GAPDS , HEL-S-278 , HSD-35
Cellular Localization
Cytoplasm
Background
This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX116000 WB Image
Sample (10 μg of whole cell lysate)
A: adult zebrafish
10% SDS PAGE
GTX116000 diluted at 1:1000
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GTX116000 IHC-P Image
GAPDHS antibody detects GAPDHS protein at cytosol on human by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
GAPDHS antibody (GTX116000) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX116000 ICC/IF Image
Immunofluorescence analysis of methanol-fixed MCF-7, using GAPDHS(GTX116000) antibody at 1:200 dilution.
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GTX116000 WB Image
Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
GTX116000 diluted at 1:1000
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GTX116000 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX116000 diluted at 1:5000
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GTX116000 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDHS antibody (GTX116000) diluted at 1:500.
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REFERENCE
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REVIEW
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