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GARS antibody

Anti-GARS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54147
Anti-GARS antibody used in Western Blot (WB). GTX54147
Anti-GARS antibody used in IHC (Paraffin sections) (IHC-P). GTX54147
Anti-GARS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54147
Anti-GARS antibody used in IHC (Paraffin sections) (IHC-P). GTX54147

Cat. No. GTX54147

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all GARS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:4000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 60-370 of human GARS (NP_002038.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycyl-tRNA synthetase , CMT2D , DSMAV , GlyRS , HMN5 , SMAD1

Cellular Localization

Cytoplasm,Secreted

Background

This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-GARS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54147

GTX54147 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX54147 GARS antibody.
Blue : DAPI
Dilution : 1:100

Anti-GARS antibody used in Western Blot (WB). GTX54147

GTX54147 WB Image

WB analysis of various sample lysates using GTX54147 GARS antibody.
Dilution : 1:5000
Loading : 25μg per lane

Anti-GARS antibody used in IHC (Paraffin sections) (IHC-P). GTX54147

GTX54147 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX54147 GARS antibody.
Dilution : 1:100

Anti-GARS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54147

GTX54147 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX54147 GARS antibody.
Blue : DAPI
Dilution : 1:200

Anti-GARS antibody used in IHC (Paraffin sections) (IHC-P). GTX54147

GTX54147 IHC-P Image

IHC-P analysis of rat brain tissue using GTX54147 GARS antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399