GeneTex

  • Country / Location Selection

United States (US)

GCP4 antibody

Anti-GCP4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115949
Anti-GCP4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115949
Anti-GCP4 antibody used in Western Blot (WB). GTX115949

Cat. No. GTX115949

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GCP4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

NIH-3T3

Predict Reactivity

Zebrafish, Rabbit, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GCP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621536

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin gamma complex associated protein 4 , 76P , GCP-4 , GCP4 , Grip76 , MCCRP3

Cellular Localization

Centrosome

Background

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.

Database

Research Area

DATA IMAGES

Anti-GCP4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115949

GTX115949 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed 293T, using GCP4(GTX115949) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Anti-GCP4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115949

GTX115949 ICC/IF Image

GCP4 antibody detects GCP4 protein at cytoplasm and centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GCP4 protein stained by GCP4 antibody (GTX115949) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-GCP4 antibody used in Western Blot (WB). GTX115949

GTX115949 WB Image

GCP4 antibody detects TUBGCP4 protein by Western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
10 % SDS-PAGE
GCP4 antibody (GTX115949) dilution: 1:1000

REFERENCE

Application Reference

Li Z et al. Cell Death Differ 2019; (Epub) Haploinsufficiency of GCP4 induces autophagy and leads to photoreceptor degeneration due to defective spindle assembly in retina.
Application : WB, IHC-Fr
Reactivity : Human
Cota RR et al. J Cell Sci 2016; (Epub) MZT1 regulates microtubule nucleation by linking γTuRC assembly to adapter-mediated targeting and activation.
Application : WB
Reactivity : Human
Lecland N et al. Nat Cell Biol 2014; (Epub) The dynamics of microtubule minus ends in the human mitotic spindle.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for GCP4 antibody (GTX115949). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169