APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.36 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human GCSF Receptor. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11173871
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
colony stimulating factor 3 receptor , CD114 , GCSFR , SCN7
Cellular Localization
Isoform 2: Secreted , Cell membrane
Background
The protein encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variants encoding four different isoforms have been found for this gene, with three of the isoforms being membrane-bound and the other being secreted and soluble. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-GCSF Receptor antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109503](/templates/default/js/lightbox/images/loading.gif "Anti-GCSF Receptor antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109503")
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GTX109503 ICC/IF Image
GCSF Receptor antibody [N2C1], Internal detects GCSF Receptor protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GCSF Receptor stained by GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX109503 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX109503 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109503 IHC-P Image
GCSF Receptor antibody [N2C1], Internal detects GCSF Receptor protein at secreted on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for GCSF Receptor antibody [N2C1], Internal (GTX109503). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GCSF Receptor antibody [N2C1], Internal (GTX109503). Be the first to share your experience with this product.
![Anti-GCSF Receptor antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109503](/upload/website/prouct_img/small/GTX109503/GTX109503_44664_20220624_ICC_IF_22062919_663.webp "Anti-GCSF Receptor antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109503")
![Anti-GCSF Receptor antibody [N2C1], Internal used in Western Blot (WB). GTX109503](/upload/website/prouct_img/small/GTX109503/GTX109503_43712_20190927_WB_TPM_watermark_w_23060500_332.webp "Anti-GCSF Receptor antibody [N2C1], Internal used in Western Blot (WB). GTX109503")
![Anti-GCSF Receptor antibody [N2C1], Internal used in Western Blot (WB). GTX109503](/upload/website/prouct_img/small/GTX109503/GTX109503_40506_20190809_WB_B_w_23060500_167.webp "Anti-GCSF Receptor antibody [N2C1], Internal used in Western Blot (WB). GTX109503")
![Anti-GCSF Receptor antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX109503](/upload/website/prouct_img/small/GTX109503/GTX109503_40506_20141128_IHC_w_23060500_600.webp "Anti-GCSF Receptor antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX109503")
![Anti-GCSF Receptor antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109503](/upload/website/prouct_img/normal/GTX109503/GTX109503_44664_20220624_ICC_IF_22062919_663.webp "Anti-GCSF Receptor antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109503")
![Anti-GCSF Receptor antibody [N2C1], Internal used in Western Blot (WB). GTX109503](/upload/website/prouct_img/normal/GTX109503/GTX109503_43712_20190927_WB_TPM_watermark_w_23060500_332.webp "Anti-GCSF Receptor antibody [N2C1], Internal used in Western Blot (WB). GTX109503")
![Anti-GCSF Receptor antibody [N2C1], Internal used in Western Blot (WB). GTX109503](/upload/website/prouct_img/normal/GTX109503/GTX109503_40506_20190809_WB_B_w_23060500_167.webp "Anti-GCSF Receptor antibody [N2C1], Internal used in Western Blot (WB). GTX109503")
![Anti-GCSF Receptor antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX109503](/upload/website/prouct_img/normal/GTX109503/GTX109503_40506_20141128_IHC_w_23060500_600.webp "Anti-GCSF Receptor antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX109503")