APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
hMSC-3A6 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , 293T , A431 , HeLa , HepG2 , PC-12 , Rat2
Predict Reactivity
Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.51 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human GEF-H1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11177391
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Rho/Rac guanine nucleotide exchange factor 2 , GEF , GEF-H1 , GEFH1 , LFP40 , NEDMHM , P40
Cellular Localization
Cytoplasm , Cell junction , tight junction , Golgi apparatus , Spindle
Background
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX125893 WB Image
Sample (10 μg of whole cell lysate)
A: hMSC-3A6
B: siRNA of ARHGEF2/GEF-H1
8% SDS PAGE
GTX125893 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX125893 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GEF-H1(GTX125893) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX125893 WB Image
GEF-H1 antibody detects GEF-H1 protein by western blot analysis.
A. 30 μg Raw264.7 whole cell lysate/extract
B. 30 μg C2C12 whole cell lysate/extract
5% SDS-PAGE
GEF-H1 antibody (GTX125893) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX125893 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (GTX125893) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX125893 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (GTX125893) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX125893 IHC-Fr Image
GEF-H1 antibody detects GEF-H1 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse mouse cerebellum.Green: GEF-H1 stained by GEF-H1 antibody (GTX125893) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX125893 WB Image
GEF-H1 antibody detects GEF-H1 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw 264.7 whole cell lysate/extract
G. 30 μg C2Cl2 whole cell lysate/extract
5% SDS-PAGE
GEF-H1 antibody (GTX125893) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX125893 IP Image
Immunoprecipitation of GEF-H1 protein from 293T whole cell extracts using 5 μg of GEF-H1 antibody (GTX125893).
Western blot analysis was performed using GEF-H1 antibody (GTX125893).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX125893 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (GTX125893) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX125893 IHC-P Image
GEF-H1 antibody detects GEF-H1 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
GEF-H1 antibody (GTX125893) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX125893 IHC-P Image
GEF-H1 antibody detects GEF-H1 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
GEF-H1 antibody (GTX125893) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX125893 WB Image
GEF-H1 antibody detects GEF-H1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5% SDS-PAGE
GEF-H1 antibody (GTX125893) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX125893 WB Image
Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: HeLa
5% SDS PAGE
GTX125893 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX125893 ICC/IF Image
GEF-H1 antibody detects GEF-H1 protein at cytoskeleton and Golgi apparatus by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: GEF-H1 stained by GEF-H1 antibody (GTX125893) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
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GTX125893 IHC-P Image
GEF-H1 antibody detects GEF-H1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.GEF-H1 stained by GEF-H1 antibody (GTX125893) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
REVIEW
GEF-H1 antibody
Cat. No. GTX125893
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Rating
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( Average 3.5 based on 2 users reviews)
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Application
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Western Blot(WB)
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( Average 3 based on 1 users reviews)
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Immunoprecipitation(IP)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 13-Mar-2014
Application Tested :
WB
Sample Species :
Ms
Sample :
mouse fibroblast cells
Amount used :
30μg
Blocking :
3%BSA-TBS, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 17-Oct-2012
Application Tested :
IP
Sample Species :
Hu
Sample :
3A6 cells
Amount used :
700μg for IP
Primary Antibody :
1:1000, 4°C, 16Hr
Figure Note :
3ug for IP
