GeneTex
United States (US)

GFAP antibody [GA-5] (ready-to-use)

GFAP-antibody-GA-5-ready-to-use_IHC (Formalin-fixed paraffin-embedded sections)_GTX29551-1_18121410_101.jpg

Cat No. GTX29551

Host Mouse
Clonality Monoclonal
Clone Name GA-5
Isotype IgG1
Application IHC-P
Reactivity Human
APPLICATION

Application Note

This antibody is ready to use.For best results use heat mediated antigen retrieval, this can enhance staining if tissues are over-fixed.Do not use enzymatic mediated antigen retrieval. We recommend incubating sections with the antibody for 30-60 minutes at room temperature.

Calculated MW

50 kDa. ( Note )

Positive Control

Normal brain or astrocytoma

Specificity/Sensitivity

This antibody reacts with human GFAP. It shows no cross-reaction with other intermediate filaments. Anti-GFAP stains astrocytes, ependymal cells and corresponding tumors.
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3 with protein carrier and preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Antigen Species

Pig

Immunogen

GFAP isolated from porcine spinal cord.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glial Fibrillary Acidic Protein,Alxdrd,Gfap

Background

Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. Neuroblastomas, Schwannomas, as well as extra-CNS tumors are not labeled. Negative staining has been observed with lymphatic tissue, muscle, gastrointestinal tract, liver, kidney, pancreas and bladder.

Database

Research Area

DATA IMAGES
GFAP-antibody-GA-5-ready-to-use_IHC (Formalin-fixed paraffin-embedded sections)_GTX29551-1_18121410_101.jpg

GTX29551 IHC-P Image

Formalin fixed paraffin embedded human brain stained with GFAP antibody (cat. # GTX73615).

Package List Price ($)
$ 319