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GFAP antibody

Anti-GFAP antibody used in Western Blot (WB). GTX27260
Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27260
Anti-GFAP antibody used in Western Blot (WB). GTX27260
Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27260
Anti-GFAP antibody used in Western Blot (WB). GTX27260

Cat. No. GTX27260

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish, Rabbit, Bovine, Dog, Chicken, Guinea pig, Pig, Horse
Package
50 μl ($399)
See all GFAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000 - 1:10000
ICC/IF 1:1000 - 1:5000
IHC-P 1:1000 - 1:5000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

5mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Bovine

Immunogen

Recombinant full-length human GFAP isotype 1 expressed in and purified from E. coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_370784

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glial fibrillary acidic protein

Database

Research Area

DATA IMAGES

Anti-GFAP antibody used in Western Blot (WB). GTX27260

GTX27260 WB Image

WB analysis of human brain tissue lysate using GTX27260 GFAP antibody.

Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27260

GTX27260 ICC/IF Image

ICC/IF analysis of rat neuron cells using GTX27260 GFAP antibody.
Red : Neurofilament Heavy antibody
Green : GFAP antibody

Anti-GFAP antibody used in Western Blot (WB). GTX27260

GTX27260 WB Image

WB analysis of rat brain tissue lysate using GTX27260 GFAP antibody.
Dilution : 1:5000

Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27260

GTX27260 ICC/IF Image

ICC/IF analysis of mixed neuron/glia cell cultures using GTX27260 GFAP antibody. The fibroblastic cells contain only Vimentin and so are green. The astrocytes contain either Vimentin and GFAP (appearing golden) or predominantly GFAP (appearing red).
Red : GFAP antibody
Green : Vimentin antibody
Blue : DAPI

Anti-GFAP antibody used in Western Blot (WB). GTX27260

GTX27260 WB Image

WB analysis of rat cerebellum tissue using GTX27260 GFAP antibody.

REFERENCE

Application Reference

Chung-Min Tsai et al. Neurophotonics 2022; 9 (1) : 015006 Transcranial photobiomodulation (808?nm) attenuates pentylenetetrazole-induced seizures by suppressing hippocampal neuroinflammation, astrogliosis, and microgliosis in peripubertal rats.
Meritet DM et al. Zebrafish 2019; 16 (3) : 280-290 Evaluating the Effects of Various Decalcification Protocols on Immunohistochemical Staining in Zebrafish (Danio rerio).
Application : IHC-P
Reactivity : Zebrafish
Sfacteria A et al. Vet Q 2015; : 43104 Peripheral nerve sheath tumor invading the nasal cavities of a 6-year-old female Pointer dog.
Reactivity : Dog
Marino F et al. J Fish Dis 2012; 35 (3) : 239-42 Spontaneous schwannoma in zebrafish, Danio rerio (Hamilton).
Application : IHC-P
Reactivity : Zebrafish
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Package List Price ($)
$ 399