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GFAP (phospho Ser38) antibody

Anti-GFAP (phospho Ser38) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55065
Anti-GFAP (phospho Ser38) antibody used in IHC (Paraffin sections) (IHC-P). GTX55065
Anti-GFAP (phospho Ser38) antibody used in Western Blot (WB). GTX55065

Cat. No. GTX55065

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all GFAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of GFAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glial fibrillary acidic protein , ALXDRD

Cellular Localization

Cytoplasm

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-GFAP (phospho Ser38) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55065

GTX55065 ICC/IF Image

ICC/IF analysis of formalin-fixed COLO205 cells using GTX55065 GFAP (phospho Ser38) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-GFAP (phospho Ser38) antibody used in IHC (Paraffin sections) (IHC-P). GTX55065

GTX55065 IHC-P Image

IHC-P analysis of formalin fixed human brain tissue section using GTX55065 GFAP (phospho Ser38) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-GFAP (phospho Ser38) antibody used in Western Blot (WB). GTX55065

GTX55065 WB Image

WB analysis of COLO205 (A), mouse brain (B) whole cell lysates using GTX55065 GFAP (phospho Ser38) antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399