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GIP antibody

Overexpression
Anti-GIP antibody used in IHC (Paraffin sections) (IHC-P). GTX55639
Anti-GIP antibody used in Western Blot (WB). GTX55639
Anti-GIP antibody used in Western Blot (WB). GTX55639

Cat. No. GTX55639

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Golden Syrian Hamster
Package
100 μl ($399)
See all GIP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 22-153 of human GIP (NP_004114.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gastric inhibitory polypeptide

Cellular Localization

Secreted

Background

This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GIP antibody used in IHC (Paraffin sections) (IHC-P). GTX55639

GTX55639 IHC-P Image

IHC-P analysis of mouse intestine tissue using GTX55639 GIP antibody.
Antigen retrieval : 10 mM citrate buffer pH 6.0
Dilution : 1:50

Anti-GIP antibody used in Western Blot (WB). GTX55639

GTX55639 WB Image

WB analysis of mouse small intestine tissue lysate using GTX55639 GIP antibody.
Loading : 25 μg
Dilution : 1:500

Anti-GIP antibody used in Western Blot (WB). GTX55639

GTX55639 WB Image

Overexpression

The data was published in the 2022 in Antioxidants (Basel). PMID: 35204073

REFERENCE

Application Reference

Jiwon Ko et al. Antioxidants (Basel) 2022; 11 (2) Protective Effect of GIP against Monosodium Glutamate-Induced Ferroptosis in Mouse Hippocampal HT-22 Cells through the MAPK Signaling Pathway.
Application : WB
Reactivity : Golden Syrian Hamster
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399