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GLE1 antibody

Anti-GLE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107056
Anti-GLE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107056
Anti-GLE1 antibody used in Western Blot (WB). GTX107056
Anti-GLE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107056

Cat No. GTX107056

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

293T , A431 , HeLa , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GLE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Gle1, Rna Export Mediator,Gle1L,Lccs,Lccs1,Hgle1,Gle1

Cellular Localization

Nucleus , Cytoplasm , Isoform 1: Cytoplasm , nuclear pore complex

Background

This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
Anti-GLE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107056

GTX107056 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GLE1(GTX107056) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-GLE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107056

GTX107056 IHC-P Image

GLE1 antibody detects GLE1 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GLE1 antibody (GTX107056) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GLE1 antibody used in Western Blot (WB). GTX107056

GTX107056 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX107056 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GLE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107056

GTX107056 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using GLE1(GTX107056) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169