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GLUT4 antibody [3G10A3]

Anti-GLUT4 antibody [3G10A3] used in IHC (Paraffin sections) (IHC-P). GTX60674
Anti-GLUT4 antibody [3G10A3] used in Flow cytometry (FACS). GTX60674
Anti-GLUT4 antibody [3G10A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60674
Anti-GLUT4 antibody [3G10A3] used in IHC (Paraffin sections) (IHC-P). GTX60674
Anti-GLUT4 antibody [3G10A3] used in Western Blot (WB). GTX60674
Anti-GLUT4 antibody [3G10A3] used in Western Blot (WB). GTX60674
Anti-GLUT4 antibody [3G10A3] used in ELISA (ELISA). GTX60674
Anti-GLUT4 antibody [3G10A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60674

Cat. No. GTX60674

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G10A3

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all GLUT4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human GLUT4 (AA: 224-353 ) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887998

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 2 member 4 , GLUT4

Cellular Localization

Cell membrane

Background

This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GLUT4 antibody [3G10A3] used in IHC (Paraffin sections) (IHC-P). GTX60674

GTX60674 IHC-P Image

IHC-P analysis of human cardiac muscle tissue using GTX60674 GLUT4 antibody [3G10A3].

Anti-GLUT4 antibody [3G10A3] used in Flow cytometry (FACS). GTX60674

GTX60674 FACS Image

FACS analysis of HeLa cells using GTX60674 GLUT4 antibody [3G10A3].
Green : GLUT4
Purple : negative control

Anti-GLUT4 antibody [3G10A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60674

GTX60674 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60674 GLUT4 antibody [3G10A3].
Green : GLUT4
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-GLUT4 antibody [3G10A3] used in IHC (Paraffin sections) (IHC-P). GTX60674

GTX60674 IHC-P Image

IHC-P analysis of bladder cancer tissue using GTX60674 GLUT4 antibody [3G10A3].

Anti-GLUT4 antibody [3G10A3] used in Western Blot (WB). GTX60674

GTX60674 WB Image

WB analysis of HeLa (1), NIH3T3 (2), 3T3-L1 (3) cell and Mouse heart (4) tissue lysate using GTX60674 GLUT4 antibody [3G10A3].

Anti-GLUT4 antibody [3G10A3] used in Western Blot (WB). GTX60674

GTX60674 WB Image

WB analysis of HEK293 (1) and GLUT4 (AA: 224-353)-hIgGFc transfected HEK293 (2) cell lysate using GTX60674 GLUT4 antibody [3G10A3].

Anti-GLUT4 antibody [3G10A3] used in ELISA (ELISA). GTX60674

GTX60674 ELISA Image

ELISA analysis of antigen using GTX60674 GLUT4 antibody [3G10A3].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-GLUT4 antibody [3G10A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60674

GTX60674 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60674 GLUT4 antibody [3G10A3].
Green : GLUT4
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

Application Reference

Lammi C et al. Int J Mol Sci 2015; 16 (11) : 27362-70 Three Peptides from Soy Glycinin Modulate Glucose Metabolism in Human Hepatic HepG2 Cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399