GeneTex

  • Country / Location Selection

United States (US)

GNAT1 antibody [N1C3]

Anti-GNAT1 antibody [N1C3] used in IHC (Frozen sections) (IHC-Fr). GTX105960
Anti-GNAT1 antibody [N1C3] used in Western Blot (WB). GTX105960

Cat. No. GTX105960

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all GNAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Predict Reactivity

Human, Rat, Zebrafish, Bovine, Dog, Chicken, Guinea pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GNAT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174639

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein subunit alpha transducin 1 , CSNB1G , CSNBAD3 , GBT1 , GNATR

Background

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GNAT1 antibody [N1C3] used in IHC (Frozen sections) (IHC-Fr). GTX105960

GTX105960 IHC-Fr Image

GNAT1 antibody [N1C3] detects GNAT1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: GNAT1 protein stained by GNAT1 antibody [N1C3] (GTX105960) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-GNAT1 antibody [N1C3] used in Western Blot (WB). GTX105960

GTX105960 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [N1C3] (GTX105960) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Stofkova A et al. Int J Mol Sci 2021; 23 (1) Depletion of Retinal Dopaminergic Activity in a Mouse Model of Rod Dysfunction Exacerbates Experimental Autoimmune Uveoretinitis: A Role for the Gateway Reflex.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for GNAT1 antibody [N1C3] (GTX105960). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169