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GOLPH2 antibody

KO/KD Orthogonal
Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154
Anti-GOLPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116154
Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154
Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154
Anti-GOLPH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116154
Anti-GOLPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116154

Cat. No. GTX116154

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GOLPH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

DU145 , HeLa membrane extract , 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GOLPH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171591

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

golgi membrane protein 1 , C9orf155 , GOLPH2 , GP73 , HEL46 , PSEC0257 , bA379P1.3

Cellular Localization

Golgi apparatus , cis-Golgi network membrane; Single-pass type II membrane protein

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this encoded protein has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154

GTX116154 WB Image

KO/KD

Wild-type (WT) and GOLPH2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody (GTX116154) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GOLPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116154

GTX116154 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GOLPH2(GTX116154) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154

GTX116154 WB Image

Orthogonal

HeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody (GTX116154) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154

GTX116154 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody (GTX116154) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GOLPH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116154

GTX116154 IHC-P Image

GOLPH2 antibody detects GOLPH2 protein at Golgi apparatus by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
GOLPH2 stained by GOLPH2 antibody (GTX116154) diluted at 1:10000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GOLPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116154

GTX116154 ICC/IF Image

GOLPH2 antibody detects GOLPH2 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GOLPH2 stained by GOLPH2 antibody (GTX116154) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE

Application Reference

Truberg J et al. Biochim Biophys Acta Mol Cell Res 2022; 1869 (11) : 119345 Endogenous tagging reveals a mid-Golgi localization of the glycosyltransferase-cleaving intramembrane protease SPPL3
Application : WB
Reactivity : Human
Yoon MJ et al. Carcinogenesis 2013; 34 (8) : 1918-28 Monensin, a polyether ionophore antibiotic, overcomes TRAIL resistance in glioma cells via endoplasmic reticulum stress, DR5 upregulation and c-FLIP downregulation.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

GOLPH2 antibody Cat. No. GTX116154

Rating
( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-GOLPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116154
Date : Anonymous submitted on 27-Oct-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : RPE cells
Blocking : 0.2% BSA and 0.1% saponin in PBS, 25°C, 0.5Hr
Fixation : 4% paraformaldehyde, 0.3Hr
Primary Antibody : 1:100, 4°C, 16Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169