GPR30 antibody [C2C3], C-term

Name: GPR30 antibody [C2C3], C-term
Brand: GeneTex
SKU: GTX107748
Price: 399.00 USD

Anti-GPR30 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107748

Anti-GPR30 antibody [C2C3], C-term used in Western Blot (WB). GTX107748

Anti-GPR30 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107748

Cat. No. GTX107748

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all GPR30 products

View product citations for antibody GTX107748 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
Note : WB As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Product Note

Knockdown/Knockout validation was supported by references (PMID:24039841)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human GPR30. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240855

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein-coupled estrogen receptor 1 , CEPR , CMKRL2 , DRY12 , FEG-1 , GPCR-Br , GPER , GPR30 , LERGU , LERGU2 , LyGPR , mER

Cellular Localization

Cell membrane; Multi-pass membrane protein , Endoplasmic reticulum membrane; Multi-pass membrane protein , Golgi apparatus membrane; Multi-pass membrane protein

Background

This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq]

Database

Gene ID: 2852 GPER1
UniProt: Q99527 GPER1

Research Area

DATA IMAGES

GTX107748 IHC-P Image

GPR30 antibody [C2C3], C-term detects GPR30 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
GPR30 stained by GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107748 IHC-P Image

GPR30 antibody [C2C3], C-term detects GPR30 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
GPR30 stained by GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107748 WB Image

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107748 ICC/IF Image

GPR30 antibody detects GPR30 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GPR30 stained by GPR30 antibody (GTX107748) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX107748 IHC-P Image

GPR30 antibody [C2C3], C-term detects GPR30 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
GPR30 stained by GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107748 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human endometrial, using GPR30(GTX107748) antibody at 1:150 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107748 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human endometrial, using GPR30(GTX107748) antibody at 1:150 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min