GeneTex
United States (US)

GPR48 antibody [C2C3], C-term

GTX100370_39603_IHC_19011506_530.jpg
GTX100370_WB_19011506_798.jpg

Cat No. GTX100370

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , Raji

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 50% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPR48. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Leucine Rich Repeat Containing G Protein-Coupled Receptor 4,Bnmd17,Gpr48,Lgr4

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

G protein-coupled receptors (GPCRs) play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain (Weng et al., 2008 [PubMed 18424556]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX100370_39603_IHC_19011506_530.jpg

GTX100370 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using LGR4(GTX100370) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100370_WB_19011506_798.jpg

GTX100370 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX100370 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169