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GREM2 antibody [C1C3]

Overexpression
Anti-GREM2 antibody [C1C3] used in Western Blot (WB). GTX108414
Anti-GREM2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108414

Cat. No. GTX108414

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all GREM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Predict Reactivity

Mouse, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GREM2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950450

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gremlin 2, DAN family BMP antagonist , CKTSF1B2 , DAND3 , PRDC , STHAG9

Cellular Localization

Secreted

Background

This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GREM2 antibody [C1C3] used in Western Blot (WB). GTX108414

GTX108414 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with GREM2 antibody [C1C3] (GTX108414) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GREM2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108414

GTX108414 IHC-P Image

GREM2 antibody [C1C3] detects GREM2 protein at cytoplasm on human placenta by immunohistochemical analysis.
Sample: Paraffin-embedded placenta.
GREM2 antibody [C1C3] (GTX108414) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Hirayama S et al. FEBS Open Bio 2022; Triosephosphate isomerase of Streptococcus pneumoniae is released extracellularly by autolysis and binds to host plasminogen to promote its activation.
Application : ICC/IF
Reactivity : Rat
Kawagishi-Hotta M et al. Regen Ther 2021; 18 : 191-201 Gremlin 2 suppresses differentiation of stem/progenitor cells in the human skin.
Application : IHC-P
Reactivity : Human
Kawagishi-Hotta M et al. Regen Ther 2019; 11 : 324-330 Increase of gremlin 2 with age in human adipose-derived stromal/stem cells and its inhibitory effect on adipogenesis.
Application : IHC-P
Reactivity : Human
Bylund J et al. Stem Cells Dev 2017; (Epub) Coordinated proliferation and differentiation of human iPS cell-derived cardiac progenitor cells depends on BMP signaling regulation by Gremlin 2.
Application : ICC/IF
Reactivity : Human
Ikeda Y et al. Eur J Obstet Gynecol Reprod Biol 2016; 203 : 72-77 Effects of gremlin-2 on the transition of primordial follicles during early folliculogenesis in the human ovary.
Application : IHC-P
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169